The robust assembly of small symmetric nano-shells

Highly symmetric nano-shells are found in many biological systems, such as clathrin cages and viral shells. Several studies have shown that symmetric shells appear in nature as a result of the free energy minimization of a generic interaction between their constituent subunits. We examine the physical basis for the formation of symmetric shells, and using a minimal model we demonstrate that these structures can readily grow from identical subunits under non equilibrium conditions. Our model of nano-shell assembly shows that the spontaneous curvature regulates the size of the shell while the mechanical properties of the subunit determines the symmetry of the assembled structure. Understanding the minimum requirements for the formation of closed nano-shells is a necessary step towards engineering of nano-containers, which will have far reaching impact in both material science and medicine.Comment: 12 pages, 12 figure

Role of Genome in the Formation of Conical Retroviral Shells

Human immunodeficiency virus (HIV) capsid proteins spontaneously assemble around the genome into a protective protein shell called the capsid, which can take on a variety of shapes broadly classified as conical, cylindrical and irregular. The majority of capsids seen in in vivo studies are conical in shape, while in vitro experiments have shown a preference for cylindrical capsids. The factors involved in the selection of the unique shape of HIV capsids are not well understood, and in particular the impact of RNA on the formation of the capsid is not known. In this work, we study the role of the genome and its interaction with the capsid protein by modeling the genomic RNA through a mean-field theory. Our results show that the confinement free energy for a homopolymeric model genome confined in a conical capsid is lower than that in a cylindrical capsid, at least when the genome does not interact with the capsid, which seems to be the case in in vivo experiments. Conversely, the confinement free energy for the cylinder is lower than for a conical capsid if the genome is attracted to the capsid proteins as the in vitro experiments. Understanding the factors that contribute to the formation of conical capsids may shed light on the infectivity of HIV particles.Comment: 22 pages, 7 figures in J. Phys. Chem. B, 201

Impact of a non-uniform charge distribution on virus assembly

Many spherical viruses encapsulate their genome in protein shells with icosahedral symmetry. This process is spontaneous and driven by electrostatic interactions between positive domains on the virus coat proteins and the negative genome. We model the effect of the icosahedral charge distribution from the protein shell instead of uniform using a mean-field theory. We find that the non-uniform charge distribution strongly affects the optimal genome length, and that it can explain the experimentally observed phenomenon of overcharging of virus and virus-like particles